Synthesis and characterization of an HSP27-targeted nanoprobe for in vivo photoacoustic imaging of early nerve injury.
作者： Hongjiang Chen , Sihua Yang , Ting Zhou , Jiankun Xu , Jun Hu
DOI: 10.1016/J.NANO.2016.02.023
关键词:
摘要: Abstract Imaging is routinely used for clinical and diagnostic purposes, but techniques capable of high specificity resolution the early detection nerve injury are still limited. In this study, we found that heat shock protein 27 (HSP27) becomes highly upregulated within 3 to 7 days injury. Taking advantage expression pattern, conjugated gold nanorods (GNRs) HSP27-specific antibodies generate a nanoprobe (GNR-HSP27Abs) could be targeted site detected by near-infrared photoacoustic imaging. Notably, images acquired 12hours after local administration GNR-HSP27Abs demonstrated can distinguish between injured uninjured nerves in rats. Taken together, these findings expand application nanoprobe-targeted imaging nerves, prompt further development novel platform application.
core.ac.uk
sci-hub.se 参考文章(56)
Xinchun Li, Jun Shen, Jianyu Chen, Xinlu Wang, Qingyu Liu, Biling Liang, None, Magnetic resonance imaging evaluation of acute crush injury of rabbit sciatic nerve: correlation with histology. Canadian Association of Radiologists journal. ,vol. 59, pp. 123- 130 ,(2008)
Jian Zhang, Sihua Yang, Xuanrong Ji, Quan Zhou, Da Xing, Characterization of Lipid-Rich Aortic Plaques by Intravascular Photoacoustic Tomography: Ex Vivo and In Vivo Validation in a Rabbit Atherosclerosis Model With Histologic Correlation Journal of the American College of Cardiology. ,vol. 64, pp. 385- 390 ,(2014) , 10.1016/J.JACC.2014.04.053
Aykut Erbengi, Mustafa Berker, Mehmet Demirci, Mürvet Tuncel, Erdem Karabulut, Ergun Daglioglu, Microscopic and electrophysiological changes on regenerating sciatic nerves of rats treated with simvastatin. Folia Neuropathologica. ,vol. 48, pp. 49- 56 ,(2010)
Ralph Weissleder, A clearer vision for in vivo imaging. Nature Biotechnology. ,vol. 19, pp. 316- 317 ,(2001) , 10.1038/86684
Stefan M. Gehrig, Chris van der Poel, Timothy A. Sayer, Jonathan D. Schertzer, Darren C. Henstridge, Jarrod E. Church, Severine Lamon, Aaron P. Russell, Kay E. Davies, Mark A. Febbraio, Gordon S. Lynch, Hsp72 preserves muscle function and slows progression of severe muscular dystrophy Nature. ,vol. 484, pp. 394- 398 ,(2012) , 10.1038/NATURE10980
Xueding Wang, Yongjiang Pang, Geng Ku, George Stoica, Lihong V. Wang, Three-dimensional laser-induced photoacoustic tomography of mouse brain with the skin and skull intact Optics Letters. ,vol. 28, pp. 1739- 1741 ,(2003) , 10.1364/OL.28.001739
Zhongmin Ou, Baoyan Wu, Da Xing, Feifan Zhou, Huiying Wang, Yonghong Tang, Functional single-walled carbon nanotubes based on an integrin αvβ3 monoclonal antibody for highly efficient cancer cell targeting Nanotechnology. ,vol. 20, pp. 105102- ,(2009) , 10.1088/0957-4484/20/10/105102
Alaaldin M. Alkilany, Catherine J. Murphy, Toxicity and cellular uptake of gold nanoparticles: what we have learned so far? Journal of Nanoparticle Research. ,vol. 12, pp. 2313- 2333 ,(2010) , 10.1007/S11051-010-9911-8
Fengxian Zheng, Jun Pan, Xiaohui Yin, Jingyuan Li, Fei Wang, Lina Zhao, Simulation study on gold nanoparticle-cellular membrane complex in endocytosis process. Journal of Nanoscience and Nanotechnology. ,vol. 13, pp. 3990- 3998 ,(2013) , 10.1166/JNN.2013.7225
Bernhard F. Gibbs, Inna M. Yasinska, Luigi Calzolai, Douglas Gilliland, Vadim V. Sumbayev, Highly specific targeting of human leukocytes using gold nanoparticle-based biologically active conjugates. Journal of Biomedical Nanotechnology. ,vol. 10, pp. 1259- 1266 ,(2014) , 10.1166/JBN.2014.1807