Granulocyte Macrophage Colony-Stimulating Factor Expression by Both Renal Parenchymal and Immune Cells Mediates Murine Crescentic Glomerulonephritis

摘要: GM-CSF has previously been demonstrated to be important in crescentic glomerulonephritis (GN). As both renal parenchymal cells and infiltrating inflammatory produce GM-CSF, their separate contributions injury were investigated by creation of two different types chimeric mice: (1) GM-CSF-deficient (GM-CSF(-/-))-->wild-type (WT) chimeras with leukocytes that are unable (2) WT-->GM-CSF(-/-) deficient cell expression. Crescentic anti-glomerular basement membrane GN was induced WT, GM-CSF(-/-)-->WT chimeras, GM-CSF(-/-) mice planting an antigen (sheep globulin) glomeruli. WT developed severe GN, whereas protected from development disease. Glomerular T recruitment, CD40(+) glomerular cells, IFN-gamma TNF expression similar but significantly reduced GMCSF(-/-) mice, indicating either leukocyte or sources sufficient drive these aspects the response. Restricted revealed a major role for cell-derived minor leukocyte-derived formation cellular crescents; MHC II expression; serum creatinine; monocyte chemoattractant protein-1, vascular adhesion molecule, IL-1beta macrophage accumulation, proteinuria, interstitial infiltrate equivalent groups intermediate between GM-CSF(-/-), required full GN.