Genotipificación de Plasmodium falciparum por PCR múltiple por medio de los genes msp1, msp2 y glurp, en cuatro localidades de Colombia

摘要: Introduction. The genetic diversity of Plasmodium falciparum has been one the major obstacles for success anti-malaria drug therapy. It provides parasite an ability to evade host's immune response by generating changes in its antigenic composition and resistance antimalarial drugs. Objective. P.falciparum was characterized 4 Colombian localities through analysis polymorphic genes. Materials methods. Eighty-one samples were obtained from patients with uncomplicated P. malaria screened variants msp1, msp2 (merozoite surface proteins) glurp (glutamate-rich protein) a multiplex PCR assay. geographic regions sampled Tierralta (Cordoba), northwestern Colombia Orinoco river watershed eastern Colombia-- Inirida (Guainia), La Carpa (Guaviare), Casuarito (Vichada). Results. MAD20 variant detected all analyzed msp1 gene. For msp 2 gene, IC allelic family found 96.3% as compared 4.9% FC family. Both families showed size polymorphism bands between 467 513 basepairs (bp) 286 300 bp FC. products differing sizes gene grouped into 5 classes: I (600-699 bp) 2.5%, II (700-799 19.8%, III (800-899 72.8%, IV (900-999 1.2% V (1000-1099 3.7%. Conclusions. molecular marker did not provide information differentiating populations. more suitable studying diversity, however, further studies are required identify polymorphisms within two families. great circulating populations, suggested that this may be useful distinguishing recrudescence reinfection.