Serum-free fibroblast cell culture medium and preparation method thereof

摘要: The invention relates to a serum-free fibroblast cell culture medium and preparation method thereof, wherein the base is DMEM or mixture of F12 medium, exogenous additives comprise hormones, metal transporter, glutamine, lipid concentration agent, an antioxidant, purine, saccharide growth factor. Compared with in prior art, present has following advantages that: animal origin recombinant albumin not added so as avoid possibly-carried safe hidden danger reduce cost; suitable for primary subculture cells, good morphology maintained, status function cultured cells are similar serum group cells; adherence factor coating required, 24 h rate more than 60%, passage 80%, no difference exists between requirements multiple passages can met, conditions after 15 directly transferred into from serum-containing culture, successfully adhere extend without gradual adaptation process.