Deamination of mammalian glutamate receptor RNA by Xenopus dsRNA adenosine deaminase: similarities to in vivo RNA editing.

摘要: Double-stranded RNA (dsRNA) adenosine deaminase (dsRAD) converts adenosines to inosines within dsRNA. A great deal of evidence suggests that dsRAD or a related enzyme edits mammalian glutamate receptor mRNA in vivo. Here we map the deamination sites occur truncated receptor-B (gluR-B) after incubation with pure Xenopus dsRAD. We find remarkable similarities, as well distinct differences, between observed and reported be edited RNAs isolated from brain. For example, although at biologically relevant Q/R editing site occurs, it occurs much less frequently than this hypothesize similarities patterns exist because specificity is intrinsic involved selecting propose differences are due absence accessory factors play indirect roles vivo, such binding occluding certain dsRAD, promoting structure required for correct efficient editing. The work here also capable more selectivity previously thought; minimal number (average < = 5) were found each gluR-B RNA. speculate various structural elements (mismatches, bulges, loops) periodically interrupt base paired region