RNA editing of hepatitis delta virus antigenome by dsRNA-adenosine deaminase

摘要: Hepatitis delta virus (HDV) is a subviral human pathogen that requires hepatitis B (HBV) for packaging. Concurrent infection by HBV and HDV increases the risk of severe liver disease compared to with alone. The genome closed circular RNA about 1,700 bases which replicated through an intermediate, antigenome. Both RNAs can be folded into highly base-paired, rod-shaped structures, similar plant viroid RNAs. Two forms sole protein, antigen, are derived from single open reading frame editing; enzymes responsible editing have not been characterized. Here we report purified enzyme dsRAD (for double-stranded-RNA-adenosine deaminase) edit antigenomic in vitro. Most important, observe mutations critical sequences antigenome identical effects on vitro vivo editing, suggesting dsRAD, or closely related enzyme, vivo.