Editing on the genomic RNA of human hepatitis delta virus.

摘要: It has been shown previously that during replication of the genome human hepatitis delta virus (HDV), a specific nucleotide change occurs to eliminate termination codon for small antigen (G. Luo, M. Chao, S.-Y. Hsieh, C. Sureau, K. Nishikura, and J. Taylor, Virol. 64:1021-1027, 1990). This creates an extension in length open reading frame from 195 214 amino acids. These two proteins, large antigens, have important distinct roles life cycle HDV. To further investigate mechanism this alteration, we developed sensitive assay involving polymerase chain reaction monitor changes on HDV RNA sequences as they occurred transfected cells. We found substrate sequence was viral genomic rather than antigenomic RNA. independently or presence antigen. Less full-length could act substrate, but only if it also contained corresponding other side rodlike structure, which is characteristic were able reproduce base vitro, by addition purified nuclear extracts cells variety species.