Molecular cloning of hepatitis delta virus RNA from an infected woodchuck liver: sequence, structure, and applications.
作者： M Y Kuo , J Goldberg , L Coates , W Mason , J Gerin
DOI: 10.1128/JVI.62.6.1855-1861.1988
关键词: Hepatitis delta Antigens 、 Biology 、 RNA 、 Complementary DNA 、 Circular RNA 、 Virology 、 Fusion protein 、 Gene 、 Base pair 、 Virus 、 Molecular biology 、 Immunology 、 Insect Science 、 Microbiology
摘要: cDNA prepared from the single-stranded circular RNA genome of hepatitis delta virus was cloned in lambda gt11 by using liver an infected woodchuck. From sequence overlapping clones, we assembled full 1,679 nucleotides. The indicated exceptional ability for intramolecular base pairing, yielding a rod structure with at least 70% bases paired and predicted free energy -805 kcal (-3,368 kJ)/mol. Three clones contained sequences that were not only expressed as fusion proteins beta-galactosidase but recognized human virus-specific antibody. These sequenced so to establish reading frame antigen on antigenomic strand. protein produced one clone purified immunoaffinity chromatography then used raise rabbit antibodies specific antigen.
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