A Novel Platelet-Rich Arterial Thrombosis Model in Rabbits

摘要: Abstract Though numerous animal thrombosis models have been introduced, an easy, reliable, and reproducible arterial model remains a continuing challenge prior to thrombolytic study. In effort evaluate the efficiency of various recombinant agents with specific affinity activated platelets in vivo, we developed novel double-opposing inverted-sutures create platelet-rich thrombus femoral artery rabbits. The arteriotomy was done semicircumferentially, variously sized microsurgical sutures were introduced intraluminally inverted manner. animals divided into three groups according used: Group 1 10-0 nylon ( n =6), 2 9-0 3 8-0 =22). superficial epigastric branch cannulated thin polyethylene (PE) tube for intraarterial administration studied agent. blood flow continuously measured real-time ultrasonic meter. Within h installation sutures, there no formation either or 2. 3, rate 91% (20 22) under steady baseline (with average 12.23±2.40 ml/min). It highly statistically significant P -value .0000743 using Fisher's Exact Test. averaged time 21.8±9.8 min. meter record dynamic measurement guideline dissolution, which correlated morphological findings stenotic status vessel detected by Doppler sonography. components proven be predominant histological examination via hematoxylin eosin (H&E) stain transmission electron microscopy (TEM). To confirm that would useful study agents, evaluated effects tissue-type plasminogen activator (rt-PA) streptokinase-human (SK-HPlg). thrombolysis post rt-PA infusion 16.0±8.2 min SK-HPlg 79.6±23.1, similar previous reports. conclusion, (8-0 nylon) provides simple, noninvasive measurement. may applied assessing offers many advantages vivo model.