Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos)

摘要: We here describe 34 novel microsatellite loci from the brown bear (Ursus arctos) identified using a next-generation sequencing approach. While we found allelic variation in 30 of these, selected 16 polymorphic microsatellites (11 di- and 5 tetranucleotide repeats) for four multiplex-PCR assays based on genetic variability, PCR performance interpretability. Applying these microsatellites, screened 31 bears northern European population. The number alleles encountered at single was between 4 10, expected observed heterozygosities ranged 0.66–0.85 0.60–0.97, respectively. Seven 120 pairwise locus combinations showed linkage disequilibrium one deviated Hardy–Weinberg equilibrium, while no null-alleles were detected. Our may be useful forensics, parentage population studies.