Detection of Campylobacter jejuni and Campylobacter coli in chicken meat samples by real-time nucleic acid sequence-based amplification with molecular beacons.
作者： E. Churruca , C. Girbau , I. Martínez , E. Mateo , R. Alonso
DOI: 10.1016/J.IJFOODMICRO.2007.02.007
关键词:
摘要: A nucleic acid sequence-based amplification (NASBA) assay based on molecular beacons was used for real-time detection of Campylobacter jejuni and coli in samples chicken meat. set specific primers beacon probe were designed to target the 16S rRNA both species. The NASBA protocol including RNA isolation valid cell suspensions buffered saline artificially contaminated meat samples. presence could be correlated with cellular viability, following inactivation bacteria by heating, inoculated but not RNase-free suspensions.
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