PIWI-interacting RNAs as novel regulators of pancreatic beta cell function
作者： Imène Sarah Henaoui , Cécile Jacovetti , Inês Guerra Mollet , Claudiane Guay , Jonathan Sobel
DOI: 10.1007/S00125-017-4368-2
关键词:
摘要: P-element induced Wimpy testis (PIWI)-interacting RNAs (piRNAs) are small non-coding that interact with PIWI proteins and guide them to silence transposable elements. They abundantly expressed in germline cells play key roles spermatogenesis. There is mounting evidence piRNAs also present somatic cells, where they may accomplish additional regulatory tasks. The aim of this study was identify the pancreatic islets determine whether involved control beta cell activities. piRNA profiling rat performed by microarray analysis. functions were investigated silencing two main Piwi genes or modulating level selected islet cells. We detected about 18,000 islets, many which differentially throughout postnatal development. Moreover, we identified changes several Goto–Kakizaki rats, a well-established animal model type 2 diabetes. Silencing Piwil2 Piwil4 adult caused reduction resulted defective insulin secretion increased resistance cytokine-induced death. Furthermore, overexpression animals upregulated diabetic rats led selective defect glucose-induced release. Our results provide for role their associated functions, suggest possible involvement development Data have been deposited Gene Expression Omnibus repository under accession number GSE93792. can be accessed via following link: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=ojklueugdzehpkv&acc=GSE93792
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