The aminoterminus of c-Raf-1 binds a protein kinase phosphorylating Ser259.

摘要: The kinase negative aminoterminal domain of c-Raf-1 expressed as glutathione S-transferase fusion protein was phosphorylated in vitro after treatment with lysates from A431 cells and subsequent assay. This phosphorylation independent stimulation the EGF; it occurred exclusively on serine mapped to Ser259. identical site by metabolic labelling vivo. binding various GST-Raf deletion mutants aminoacid residues 181 255.