Optimization of printing buffer for protein microarrays based on aldehyde-modified glass slides

摘要: It is of great importance to efficiently immobilize probes onto a substrate with good spot quality for fabrication protein microarrays. Printing buffers play an essential role in the process In this work, antigen (Ag)/antibody (Ab) microarrays were fabricated on 3-aminopropyltriethoxysilane (APTES) modified glass slides through glutaraldehyde (GA), bis-aldehyde homobifunctional cross-linker. Different types such as triton X-100 and glycerol their effects immobilization investigated improving microspots efficiency aldehyde-activated APTES silanized slides. addition, performance optimized printing buffer was characterized Ag/Ab The results indicated that buffer, 0.01 M PBS additional 0.003% 10% could effectively eliminate non-homogeneous morphology significantly improve signal intensities. provide improved approach construct high