Proteomic approaches in biological and medical sciences: principles and applications.

摘要: After the first introduction of concept "proteome" more than 10 years ago, large-scale studies protein expression, localization, activities and interactions have gained an exponential increase interest, leading to extensive research technology development. Proteomics is expansively applied in many areas, ranging from basic research, various disease malignant tumors diagnostic biomarker discovery therapeutic applications. Several proteomics approaches been developed for separation identification, characterization function structure. Two-dimensional gel electrophoresis, chromatography, capillary electrophoresis mass spectrometry become most used methods. These techniques are also under constant This review provides overview main their combinations, proteomics. The emphasis made on description advantages disadvantages each technique, navigate selection best application solving a specific problem.