Comparative Proteomics: An Approach to Elucidating the Function of a Novel Gene Called BRE

摘要: Proteomics was developed in the early 1990s to allow proteins expressed by cells and tissues be systematically studied (Celis et al., 1999; Arrell 2001). The word proteome coined Marc Wilkins al (Wilkins al, 1996) from words “protein genome”. It is therefore defined as protein equivalent of genome. Generally, unique spectrum only synthesized specific cell types, for example amylase secreted parotid gland, insulin pancreas thyroxin thyroid follicles. Protein synthesis a complicated process formed different combination length 20 amino acids found our body (Arnstein, 1965). For example, following transcription genes encoded DNA, mRNAs translocate into cytoplasm where they are translated type ribosomes (Lengyel, 1966). This then followed post-translational modification peptide chain configure so that it becomes biologically active. Post-translational modifications involve glycosylation, alkylation, methylation sulfation (Blundell 1993, Fleischer, 1983). coand transported during cellular homeostasis (Finnerty 1979; Mao 2011). In this chapter, we have described comparative 2-dimensional electrophoresis (2-DE) proteomics workflow identification mass spectometry. Comparative used