Automated instrumentation for comprehensive two-dimensional high-performance liquid chromatography of proteins

作者: Michelle M. Bushey , James W. Jorgenson

DOI: 10.1021/AC00201A015

关键词: ChemistryAnalytical chemistryElutionColumn (database)High-performance liquid chromatographySize-exclusion chromatographyInstrumentationChromatographyDimension (vector space)Contour mappingChromatographic separation

摘要: A comprehensive two-dimensional (2-D) liquid chromatographic separation system Is presented. The system uses a microbore cation exchange column, operated under gradient …

参考文章(23)
Nobuhiro Takahashi, Yoko Takahashi, Frank W. Putnam, Two-dimensional high-performance liquid chromatography and chemical modification in the strategy of sequence analysis Journal of Chromatography A. ,vol. 266, pp. 511- 522 ,(1983) , 10.1016/S0021-9673(01)90922-7
E.L. Johnson, R. Gloor, Ronald E. Majors, Coupled column chromatography employing exclusion and a reversed phase Journal of Chromatography A. ,vol. 149, pp. 571- 585 ,(1978) , 10.1016/S0021-9673(00)81012-2
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
G. Guiochon, L.A. Beaver, M.F. Gonnord, A.M. Siouffi, M. Zakaria, Theoretical investigation of the potentialities of the use of a multidimensional column in chromatography Journal of Chromatography A. ,vol. 255, pp. 415- 437 ,(1983) , 10.1016/S0021-9673(01)88298-4
F. Erni, R.W. Frei, Two-dimensional column liquid chromatographic technique for resolution of complex mixtures Journal of Chromatography A. ,vol. 149, pp. 561- 569 ,(1978) , 10.1016/S0021-9673(00)81011-0
D.S. Gough, P. Hannaford, A. Walsh, The application of cathodic sputtering to the production of atomic vapours in atomic fluorescence spectroscopy Spectrochimica Acta Part B: Atomic Spectroscopy. ,vol. 28, pp. 197- 210 ,(1973) , 10.1016/0584-8547(73)80024-2
B. W. Smith, J. B. Womack, N. Omenetto, J. D. Winefordner, Approaching Single Atom Detection with Atomic Fluorescence in a Glow Discharge Atom Reservoir Applied Spectroscopy. ,vol. 43, pp. 873- 876 ,(1989) , 10.1366/0003702894202148
D. S. Gough, J. R. Meldrum, A non-dispersive atomic fluorescence spectrometer for the direct determination of metals Analytical Chemistry. ,vol. 52, pp. 642- 646 ,(1980) , 10.1021/AC50054A011