miR-28-3p Is a Cellular Restriction Factor That Inhibits Human T Cell Leukemia Virus, Type 1 (HTLV-1) Replication and Virus Infection

摘要: Human T cell leukemia virus, type 1 (HTLV-1) replication and spread are controlled by different viral cellular factors. Although several anti-HIV microRNAs have been described, such a regulation for HTLV-1 has not reported. In this study, we found that miR-28-3p inhibits virus expression its targeting specific site within the genomic gag/pol mRNA. Because is highly expressed in resting cells, which resistant to infection, investigated potential protective role of against de novo infection. To end, developed new sensitive quantitative assay on basis detection products reverse transcription. We demonstrate does prevent receptor interaction or entry but, instead, induces post-entry block at transcription level. addition, HTLV-1, subtype 1A isolates corresponding Japanese strain ATK-1 present natural, single-nucleotide polymorphism target site. As result polymorphism, sequence was inhibited miR-28. Interestingly, genetic studies transmission shown strain, carries Thr-to-Cys transition mutation, transmitted efficiently between spouses, suggesting miR-28 may play an important transmission.