Embryogenesis in vitro of several Citrus species and cultivars

摘要: SummaryA number of experiments were performed to obtain somatic embryos and embryogenie cell lines from several species cultivars citrus by in vitro ovule culture. The source ovules (pre-anthesis post-anthesis), incubation conditions (light darkness) modifications the culture medium evaluated. All assayed produced “pseudobulbi”, but production friable callus was dependent. small proliferation globular bodies recovered primary cultures suitable for recovering periodical subculturing into fresh medium. After monthly subcultures discarding virtually devoid organized structures obtained three sweet orange, sour orange Cleopatra mandarin. In all these species, embryogenesis restored spontaneously and/or substitution sucrose maltose or lactose m...