recA-Based PCR Assay for Accurate Differentiation of Streptococcus pneumoniae from Other Viridans Streptococci
作者： A. Zbinden , N. Kohler , G. V. Bloemberg
DOI: 10.1128/JCM.01450-10
关键词:
摘要: Proper identification of Streptococcus pneumoniae by conventional methods remains problematic. The discriminatory power the 16S rRNA gene, which can be considered "gold standard" for molecular identification, is too low to differentiate S. from closely related species such as pseudopneumoniae, mitis, and oralis in routine clinical laboratory. A 313-bp part recA was selected on basis variability within mitis group, showing <95.8% interspecies homology. In addition, 6 signature nucleotides specific were identified fragment. We show that analysis a useful tool proper level particular, pneumococci.
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