Proteolytic activity and expression of the 20S proteasome are increased in psoriasis lesional skin.

摘要: Summary Background  Deregulation of the proteasome pathway has been shown to be involved in pathogenesis several inflammatory disorders. To date limited information exists on and immunoproteasome expression activity psoriasis skin. Objectives  investigate potential role proteasomes psoriasis. Methods  Thirty-six patients with 40 healthy subjects were included. The protein mRNA levels proteolytic subunits determined using immunohistochemistry, quantitative polymerase chain reaction fluorogenic peptide substrate lesional nonlesional skin. We additionally measured plasmatic (p-proteasome) enzyme-linked immunosorbent assay. Results  reveal an increased but stable skin as compared (n = 19), suggesting that is regulated post-transcriptionally. This overexpression was associated a significant increase proteasomal chymotrypsin-like threefold higher than (n = 3). p-Proteasome enhanced (mean ± SEM 3960 ± 299 ng mL−1, range 1484–8987) when controls (2535 ± 187 ng mL−1, 654–6446, P < 0·001) significantly psoriatic arthritis (4937 ± 572 ng mL−1, 2600–8987). In addition, they correlated body surface area appeared thus new biomarker severity. Conclusions  Altogether these results strongly suggest involvement system support relevance inhibitors local or systemic treatment psoriasis.