ECTOCELLULAR GLUCOSIDASE AND PEPTIDASE ACTIVITY OF THE MIXOTROPHIC DINOFLAGELLATE PROROCENTRUM MINIMUM (DINOPHYCEAE)(1).
作者： Monica Salerno , Diane K. Stoecker
DOI: 10.1111/J.1529-8817.2008.00622.X
关键词:
摘要: The activities of the enzymes α- and β-glucosidase, leucine aminopeptidase were measured in cultures dinoflagellate Prorocentrum minimum (Pavill.) J. Schiller field samples collected during blooms occurring tributaries Chesapeake Bay, Maryland, USA. Activities using fluorogenic artificial substrates partitioned among >5 μm size fraction, small microbes fraction (0.1-5 μm), dissolved phase ( 5 μm thus can be separated from which contains most bacteria. Little to no glucosidase activity was detected associated with or samples, (67% 93% cultures, 54% 100% samples) for both α β glucosidase. In contrast, 67% 90% total (LAP) fraction. Within a culture, LAP containing P. decreased response ammonium urea additions, but not nitrate. positively correlated abundance chl a, negatively concentration. During blooms, up 34% indicating that when abundant, dinoflagellates may make substantial contribution ectocellular water column.
sci-hub.se 参考文章(58)
Gilles Billen, Protein Degradation in Aquatic Environments Microbial Enzymes in Aquatic Environments. pp. 123- 143 ,(1991) , 10.1007/978-1-4612-3090-8_7
F. Azam, D. C. Smith, Bacterial Influence on the Variability in the Ocean’s Biogeochemical State: A Mechanistic View Springer Berlin Heidelberg. pp. 213- 236 ,(1991) , 10.1007/978-3-642-75121-9_9
Ryszard J. Chróst, Environmental Control of the Synthesis and Activity of Aquatic Microbial Ectoenzymes Microbial Enzymes in Aquatic Environments. pp. 29- 59 ,(1991) , 10.1007/978-1-4612-3090-8_3
D.J. Gifford, D.A. Caron, Sampling, preservation, enumeration and biomass of marine protozooplankton ICES Zooplankton Methodology Manual. pp. 193- 221 ,(2000) , 10.1016/B978-012327645-2/50006-2
Ronald Benner, Chapter 3 – Chemical Composition and Reactivity Biogeochemistry of Marine Dissolved Organic Matter. pp. 59- 90 ,(2002) , 10.1016/B978-012323841-2/50005-1
D.M. Karl, K.M. Björkman, Chapter 6 – Dynamics of DOP Biogeochemistry of Marine Dissolved Organic Matter. pp. 249- 266 ,(2002) , 10.1016/B978-012323841-2/50008-7
Roger Harris, Peter Wiebe, Jurgen Lenz, Hein-Rune Skjoldal, Mark Huntley, ICES zooplankton methodology manual Academic. ,(2000)
Hans-Georg Hoppe, Sang-Jin Kim, Klaus Gocke, Microbial Decomposition in Aquatic Environments: Combined Process of Extracellular Enzyme Activity and Substrate Uptake Applied and Environmental Microbiology. ,vol. 54, pp. 784- 790 ,(1988) , 10.1128/AEM.54.3.784-790.1988
P. M. Glibert, C. Legrand, The Diverse Nutrient Strategies of Harmful Algae: Focus on Osmotrophy Ecological Studies. pp. 163- 175 ,(2006) , 10.1007/978-3-540-32210-8_13
Craig A. Carlson, Dennis A. Hansell, Biogeochemistry of marine dissolved organic matter Academic Press. ,(2015)