Analysis of corneal and conjunctival microenvironments using monoclonal antibodies

摘要: PURPOSE The authors phenotypically compared epithelial and nonepithelial components of human corneal conjunctival microenvironments using a panel monoclonal antibody reagents that included markers cell maturation, mesodermal-derived fibrous tissue vessels, specific keratins, major histocompatibility complex Class I II antigens. METHODS Corneoscleral rims obtained after trephination the donor button for use in penetrating keratoplasty procedures were studied. RESULTS A comparison cornea conjunctiva with anti-epithelial antikeratin antibodies demonstrated keratinocytes undergo similar antigenically defined pathways maturation. However, reactivity 12/1-2 (antibody against low molecular weight keratins) but not basal cells suggested differences keratin expression between two types. Using antigens, it was tissues determinants found on all epithelial, stromal, endothelial cells, Langerhans' subset stromal cells. CONCLUSIONS availability tissue-specific should be study roles ocular microenvironment might play pathogenesis inflammatory diseases.