Comparison of cyclic nucleotide phosphodiesterase isoforms from rat heart and bovine aorta. Separation and inhibition by selective reference phosphodiesterase inhibitors.
作者： A.F. Prigent , S. Fougier , G. Nemoz , G. Anker , H. Pacheco
DOI: 10.1016/0006-2952(88)90400-5
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摘要: Abstract The resolution as well the biochemical properties of multiple molecular forms cyclic nucleotide phosphodiesterase, in a given tissue, may be strongly dependent upon experimental conditions preparation (extraction crude enzyme from tissues and fractionation procedures). In present study, we compare different cardiac (rat heart ventricle) vascular (bovine aorta) phosphodiesterase isolated extracts prepared either sucrose medium or hypotonic (in presence protease inhibitors ion chelators) using two procedures: isoelectric focusing on flat gel bed DEAE-Trisacryl anion exchange chromatography. Both calmodulin-dependent cAMP-specific exhibited close IEF chromatographic patterns showed similar sensitivities towards reference regardless tissue origin. marked contrast, cGMP-specific isoform notably differed one to another with respect its (only being capable stimulation by cGMP) xenobiotics. Thus possibility exists that pharmacological agents modulate activity differently target tissues.
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