A modified assay of 3′:5′-cyclic-AMP phosphodiesterase
作者： R.J. Boudreau , G.I. Drummond
DOI: 10.1016/0003-2697(75)90361-9
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摘要: Abstract A modification of the assay cyclic nucleotide phosphodiesterase involving batch use Dowex 1 anion exchange resin is described which allows for quantitative recovery adenosine, guanosine, and their metabolites from slurry. The suitable in crude preparations containing purine catabolizing enzymes. standardized procedure determining kinetic parameters AMP hydrolysis also discussed. This was used partial characterization kinetics by rat rabbit heart supernatant fractions.
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