Fluorescence endoscopy of cathepsin activity discriminates dysplasia from colitis.

摘要: BACKGROUND Surveillance colonoscopy using random biopsies to detect colitis-associated cancer (CAC) suffers from poor sensitivity. Although chromoendoscopy improves detection, acceptance in the community has been slow. Here, we examine usefulness of near infrared fluorescence (NIRF) endoscopy image molecular probes for cathepsin activity colitis-induced dysplasia. METHODS In patient samples, was correlated with colitis and mice, detected as fluorescent hydrolysis product substrate-based after injection into Il10(-/-) colitic mice. Fluorescence colonic whole-mount imaging were performed before complete sectioning pathology review resected colons. RESULTS Cathepsin 5-fold 8-fold higher dysplasia CAC, respectively, compared areas mild tissue sections. The signal-to-noise ratios dysplastic lesions seen by mice 5.2 ± 1.3 (P = 0.0001). Lesions increased NIRF emissions classified raised or flat dysplasia, lymphoid tissue, ulcers. Using images collected endoscopy, a receiver operating characteristic curve correctly diagnosing calculated. area under 0.927. At cutoff 1000 mean intensity, sensitivity specificity detecting 100% 83%, respectively. Analysis revealed that focally enhanced derived numbers infiltrating myeloid-derived suppressor cells macrophages equivalent activity. CONCLUSIONS These studies indicate probe identifies foci within chronically inflamed Combined white light presents unique advantages may increase surveillance patients CAC.