Growth rate dependence of transfer RNA abundance in Escherichia coli.

摘要: We have tested the predictions of a model that accounts for codon preferences bacteria in terms growth maximization strategy. According to this tRNA species cognate minor and major codons should be regulated differently under different conditions: isoacceptors increase at fast rates while those decrease rates. used quantitative Northern blotting technique measure abundance methionine leucine isoacceptor families over ranging from 0.5 2.1 doublings per hour. Five are (tRNA(eMet), tRNA(1fMet), tRNA(2fMet), tRNA(1Leu) tRNA(3Leu) both as relative fraction total absolute concentration with increasing Three (tRNA(2Leu), tRNA(4Leu) tRNA(5Leu) RNA These data suggest abundances groups ways, they not simply according specificity. In particular, support optimization bias.