Human EML4, a novel member of the EMAP family, is essential for microtubule formation

摘要: Abstract Human EML4 (EMAP-like protein 4) is a novel microtubule-associated WD-repeat of 120 kDa molecular weight, which classified as belonging to the conserved family EMAP-like proteins. Cosedimentation assays demonstrated that associates with in vitro polymerized microtubules. Correspondingly, immunofluorescence stainings and transient expression EGFP-labeled revealed complete colocalization interphase microtubule array HeLa cells. We present evidence amino-terminal portion (amino acids 1–249) essential for association Immunoprecipitation experiments hyperphosphorylated on serine/threonine residues during mitosis. In addition, associated mitotic spindle, suggesting function regulated by phosphorylation. siRNA-mediated knockdown cells led significant decrease number no case figures could be observed negative Additionally, we reduction proliferation rate uptake radioactive [ 3 H]-thymidine result silencing. Most importantly, showed completely modified network, indicating necessary correct formation.