Alternative acute inhalation toxicity testing by determination of the concentration-time-mortality relationship : experimental comparison with standard LC50 testing

摘要: A new design for acute inhalation toxicity testing was evaluated and compared with results obtained according to OECD guideline 403. The consists of a range-finding test, which is compatible conventional limit can be followed by determination concentration-time-mortality relationship, enabling calculation LC50 (50% mortality exposure concentration) values. By exposing pairs rats different periods time about four test concentrations in nose-only unit, LT50 time) values were five animals per concentration. data the approximate 20 time-concentration combinations used calculate probit relationship. Estimated responses from these relations figures groups male female whole-body testing. In general, there good correspondence between estimated observed response. our hands, relationship takes same number (40-50) as procedure However, method has several additional advantages such as: (A) are over 10-fold range time, potential decreasing when regulations require exposure. (B) contains considerably more valuable information risk assessment than value. (C) less sensitive small fluctuations substance important especially substances steep concentration-response curves. this investigation indicate need reconsideration adaptation 403 on