On-line high-performance liquid chromatography : Ultraviolet-visible spectroscopy of phenolic compounds in plant extracts using post-column derivatization

摘要: Abstract High-performance liquid chromatography coupled with ultraviolet-visible spectroscopy, using a photodiode array detector, was applied to the investigation of plant extracts for polyphenolic compounds. Simultaneous detection at different wavelengths and measurement UV spectrum each separated compound during elution allow an easy rapid identification flavonoids xanthones. However, as some these compounds have closely related structures, characterization by their spectra is insufficient. Derivatization reagents inducing shift absorption maxima required furnishes additional structural information. In present work, were added eluate post-column derivatization system. The adapted in order be compatible solvent system used chromatographic separation. technique analysis crude various Gentiana species.