A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain
作者： Philippe Seguela , Ali Haghighi , Jean-Jacques Soghomonian , Ellis Cooper
DOI: 10.1523/JNEUROSCI.16-02-00448.1996
关键词:
摘要: There is strong evidence that ATP acts as an excitatory neurotransmitter in the periphery, yet little known about fast central ATP-mediated transmission. We report here molecular cloning of a novel neuronal ionotropic receptor P,, subtype (P& isolated from rat brain. This channel subunit has significant amino acid homology with two recently cloned ATP-gated channels smooth muscle (47%) and pheochromocytoma PC1 2 cells (37%). PZx3 contains characteristic 10 conserved cysteines channels, putative extracellular region homologous to Walker type A motif found various nucleotide-binding proteins, potential sites for phosphorylation by protein kinase C. Homomeric Ppx3 expressed Xenopus oocytes produce rapid cation-selective purinergic currents are potentiated zinc ions reversibly blocked antagonists suramin, Reactive Blue 2, pyridoxalphosphate-6-axophenyl-2U,4Udisulfonic acid. P,,,-receptor mRNA Purkinje granule cerebellum well CA3 pyramidal hippocampus innervated zinc-rich axon terminals mossy fibers. Our results suggest synaptic transmission mediated zinc-sensitive widespread mammalian
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