Caspase-3 can be pseudo-activated by a Ca2+-dependent proteolysis at a non-canonical site.

摘要: We have shown previously that calcium could trigger nuclear fragmentation, which was associated with a caspase 3 (C3)-like activity [Juin, P., Pelletier, M., Oliver, L., Tremblais, K., Gregoire, Meflah, K. and Vallette, F.M. (1998) Induction of caspase-3-like by in normal cytosolic extracts triggers apoptosis cell-free system. J. Biol. Chem. 273, 17559]. Here, we report this activation is non-canonical truncation C3, induces weak DEVDase activity. The cleavage C3 via calcium-dependent proteolysis independent 9; lysate exposure to prevents further the cytochrome c dATP pathway. Altogether, our data suggest favour necrotic mechanism inducing generation form insensitive mitochondrial activation.