Low-dose effect of developmental bisphenol A exposure on sperm count and behaviour in rats.

摘要: Summary Bisphenol A is widely used in food contact materials and other products detected human urine blood. Bisphenol may affect reproductive neurological development; however, opinion of the European Food Safety Authority (EFSA) on bisphenol (EFSA J, 13, 2015 3978) concluded that none available studies were robust enough to provide a point departure for setting tolerable daily intake A. In present study, pregnant Wistar rats (n = 17–21) gavaged from gestation day 7 pup 22 with doses 0, 25 μg, 250 μg, 5 mg or 50 mg/kg bw/day. offspring, growth, sexual maturation, weights histopathology organs, oestrus cyclicity sperm counts assessed. Neurobehavioural development was investigated using behavioural testing battery including tests motor activity, sweet preference, anxiety spatial learning. Decreased count found at lowest dose, 25 μg/kg/day, but not higher doses. Reproductive organ weight histology affected no effects seen male offspring. female exposure 25 μg/kg bw/day dose resulted increased body late life altered learning Morris water maze, indicating masculinization brain. sweetened females highest group, also possible sign masculinization. The endpoints significantly affected. conclusion, study experimental design, has shown developmental can cause adverse fertility (decreased count), neurodevelopment (masculinization females) lead life. These results suggest new EFSA temporary 4 μg/kg sufficiently protective regard endocrine disrupting humans.