摘要: Trimming procedures are an important part of the sequence analysis pipeline in EST Sequencing Project. In general, trimming is done several phases, each one detecting and removing some kind undesirable artifact, such as low quality sequence, vectors or adapters, contamination. However, this strategy often results a phase being unable to recognize its target because it was removed during previous phase. To remedy drawback, we propose new strategy, where detects but does not remove target, leaving decision post processing step occurring after all phases. Our tests show that can significantly improve detection artifacts.
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