Biosynthesis of monoterpenes: Hydrolysis of bornyl pyrophosphate, an essential step in camphor biosynthesis, and hydrolysis of geranyl pyrophosphate, the acyclic precursor of camphor, by enzymes from sage (Salvia officinalis)

摘要: Abstract Soluble enzyme preparations from sage ( Salvia officinalis ) leaves catalyze the hydrolysis of (+)-bornyl pyrophosphate to (+)-borneol, which is an essential step in biosynthesis cyclic monoterpene (+)-camphor [(1 R ,4 )-bornan-2-one] this tissue. Chromatography preparation on Sephadex G-150 allowed separation two regions bornyl hydrolase activity. One region was further separated into a and monophosphate by chromatography hydroxylapatite, but other contained activities were inseparable or any chromatographic technique tested. Each phosphatase pyrophosphatase activity characterized with respect molecular weight, pH optimum, response inhibitors, K m for phosphate pyrophosphate, substrate specificity, each distinctly different regard these properties. specific esters sterically hindered monoterpenols such as pyrophosphate. The preferred primary allylic alcohols geranyl neryl are precursors monoterpenes, it hydrolyzed at faster rates than similar showing preference alcohols. terpenyl exhibiting specificity may be involved camphor vivo , while more shown source potential interference studies cyclization processes.