Immune regulation of human colonic electrolyte transport in vitro.

摘要: The role of lamina propria cells in regulating human colonic ion transport was investigated vitro. Normal mucosae were mounted Ussing chambers, and short circuit current changes (delta SCC) monitored response to immune cell activation. Anti-human immunoglobulin E (anti-IgE) formyl-Methionyl-Leucyl-Phenylalanine (fMLP) used stimulate mast phagocytes respectively. Anti-IgE (100 micrograms/ml) fMLP microM) evoked rapid onset, inward delta SCC (mean (SEM) max 19.3 (2.8) 29.4 (4.7) microA/0.63 cm2 respectively). A pharmacological approach identify the charge carrying species characterise mediators involved response. Responses each secretagogue significantly attenuated by bumetanide, indicating that at least partly due electrogenic chloride secretion. Piroxicam reduced phagocyte activation 91.1 (3.4)% 48.2 (25.2)% respectively, implicating eicosanoids as responses. Mepyramine responses anti-IgE 79.6 (12.0)% but did not alter fMLP. Desensitisation repeated or stimulation, cross desensitisation between stimuli, features In summary, we have shown can Such events vivo will result gradient driven secretory diarrhoea, which may occur a protective enteric-dwelling parasites, feature local bowel inflammation.