Berberine inhibits ion transport in human colonic epithelia.

摘要: The effects of berberine on ion transport in both human colonic mucosal epithelia and an intestinal epithelial cell line (T84) were examined. Berberine (concentration range 0-500 microM) reduced basal stimulated responses mucosae a manner which was non-specific for Ca2+ -or cAMP-mediated signals. Similarly, cultured monolayers, inhibited -and indicating inhibitory activity directly at the level epithelium rather than indirect effect through other element(s). did not alter rate generation cAMP by adenylyl cyclase or protein kinase A, effector enzyme pathway. carbachol-stimulated 86Rb+ efflux from T84 monolayers. also K+ conductance apically-permeabilised re-sected mucosae. These results indicate i) that exerts anti-secretory action upon cells ii) mechanism may be blockade channels.