High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease

作者: Alan W Walker , Jeremy D Sanderson , Carol Churcher , Gareth C Parkes , Barry N Hudspith

DOI: 10.1186/1471-2180-11-7

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摘要: The gut microbiota is thought to play a key role in the development of inflammatory bowel diseases Crohn's disease (CD) and ulcerative colitis (UC). Shifts composition resident bacteria have been postulated drive chronic inflammation seen both (the "dysbiosis" hypothesis). We therefore specifically sought compare mucosa-associated from inflamed non-inflamed sites colon CD UC patients that non-IBD controls detect disease-specific profiles. Paired mucosal biopsies intestinal tissue 6 (n = 12) were compared 5 healthy 5) by in-depth sequencing over 10,000 near full-length bacterial 16S rRNA genes. results indicate microbial diversity reduced IBD, particularly CD, species disturbed. Firmicutes IBD samples there concurrent increases Bacteroidetes, only, Enterobacteriaceae. There also significant differences community structure between sites. However, these varied greatly individuals, meaning was no obvious signature positively associated with gut. These may support hypothesis overall dysbiosis observed relative might some extent be result disturbed environment rather than direct cause disease. Nonetheless, shifts important factors maintenance severity.

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