A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis
作者： J. Zhang , S. G. Van Lanen , J. Ju , W. Liu , P. C. Dorrestein
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摘要: The enediynes, unified by their unique molecular architecture and mode of action, represent some the most potent anticancer drugs ever discovered. biosynthesis enediyne core has been predicted to be initiated a polyketide synthase (PKS) that is distinct from all known PKSs. Characterization PKS involved in C-1027 (SgcE) neocarzinostatin (NcsE) now revealed (i) PKSs contain central acyl carrier protein domain C-terminal phosphopantetheinyl transferase domain; (ii) are functional heterologous hosts, coexpression with an thioesterase gene produces first isolable compound, 1,3,5,7,9,11,13-pentadecaheptaene, biosynthesis; (iii) findings for SgcE NcsE likely shared among nine-membered thereby supporting common mechanism initiate biosynthesis.
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