Fatty acid specificity of Rhizopus delemar lipase in acidolysis

摘要: Abstract We investigated the fatty acid specificity of 1,3-positional specific Rhizopus delemar lipase in acidolysis using a randomly interesterified oil, which constituent contents were approximately same, as substrate. Acidolysis was carried out at 30°C with shaking reaction mixture containing caprylic (CA)/the oil (2 : 1, w/w) and immobilized on ceramic carrier (4% mixture). The activity each experimentally determined ratio content exchanged for CA to that existing 1(3)-position substrate regarded acidolysis. hydrolysis by analysis composition acids liberated from same oil. acted strongly myristic, palmitic, palmitoleic, stearic, oleic, linoleic, α-linolenic acids, moderately arachidonic eicosapentaenoic acids. activities these relative oleic almost hydrolysis. On other hand, γ-linolenic docosahexaenoic hydrolysis, but only very weakly early stage This shows is stricter than Since kinetics borage acid-containing (TGA-25), tuna could be easily predicted acidolysis, it concluded accurately evaluated. In addition, TGA-25 completely total three cycles reaction, transesterified oils agreed 2-position oils. Therefore, shown repeated 1,3-specific applied regiospecific analysis.