One-pot enzymatic synthesis of docosahexaenoic acid-rich triacylglycerols at the sn-1(3) position using by-product from selective hydrolysis of tuna oil
作者： Toshihiro Nagao , Yomi Watanabe , Kazuaki Maruyama , Yuusuke Momokawa , Noriaki Kishimoto
DOI: 10.1016/J.NBT.2010.07.021
关键词:
摘要: Docosahexaenoic acid (DHA)-rich oil has been industrially produced by selective hydrolysis of tuna with a lipase that acts weakly on DHA. The free fatty acids (FFAs) generated in this process as by-products contain high DHA concentration (46 wt%) but are treated industrial waste. This study attempted to reuse these by-product FFAs using one-pot process, and succeeded producing triacylglycerols (TAGs) through the esterification glycerol immobilized Rhizomucor miehei lipase. Regiospecific analysis resulting TAGs showed content at sn-1(3) position (51.7 mol%) was higher than sn-2 (17.3 mol%). distribution synthesized similar from seal oil.
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