Optimization and standardization of the comet assay for analyzing the repair of DNA damage in cells

作者: Thomas Bauch , Wilfried Böcker , Ulrike Mallek , Wolfgang-Ulrich Müller , Christian Streffer

DOI: 10.1007/S000660050020

关键词:

摘要: The “comet assay” has become an interesting and a very useful tool for the analysis of induction amount DNA damage in single cells thus offering opportunity to measure effectiveness repair. On basis Ostling Johanson protocol we have developed modified method with increased sensitivity high reproducibility.

参考文章(20)
Jürgen Dunst, Susanne Neubauer, Axel Becker, Erich Gebhart, Chromosomal in-vitro radiosensitivity of lymphocytes in radiotherapy patients and AT-homozygotes. Strahlentherapie Und Onkologie. ,vol. 174, pp. 510- 516 ,(1998) , 10.1007/BF03038983
W. BOCKER, T. BAUCH, W.-U. MULLER and, Image analysis of comet assay measurements. International Journal of Radiation Biology. ,vol. 72, pp. 449- 460 ,(1997) , 10.1080/095530097143220
NP Singh, RE Stephens, EL Schneider, None, Modifications of Alkaline Microgel Electrophoresis for Sensitive Detection of DNA Damage International Journal of Radiation Biology. ,vol. 66, pp. 23- 28 ,(1994) , 10.1080/09553009414550911
S. Powell, T.J. McMillan, DNA damage and repair following treatment with ionizing radiation Radiotherapy and Oncology. ,vol. 19, pp. 95- 108 ,(1990) , 10.1016/0167-8140(90)90123-E
V.J. McKelvey-Martin, M.H.L. Green, P. Schmezer, B.L. Pool-Zobel, M.P. De Méo, A. Collins, The single cell gel electrophoresis assay (comet assay): A European review Mutation Research. ,vol. 288, pp. 47- 63 ,(1993) , 10.1016/0027-5107(93)90207-V
Daryl W. Fairbairn, Peggy L. Olive, Kim L. O'Neill, The comet assay: a comprehensive review Mutation Research\/reviews in Genetic Toxicology. ,vol. 339, pp. 37- 59 ,(1995) , 10.1016/0165-1110(94)00013-3
Maria Klaude, Stefan Eriksson, Jonas Nygren, Gunnar Ahnström, The comet assay: mechanisms and technical considerations. Mutation Research-dna Repair. ,vol. 363, pp. 89- 96 ,(1996) , 10.1016/0921-8777(95)00063-1
Catharine ML West, SAG Elyan, P Berry, R Cowan, David Scott, A comparison of the radiosensitivity of lymphocytes from normal donors, cancer patients, individuals with ataxia-telangiectasia (A-T) and A-T heterozygotes. International Journal of Radiation Biology. ,vol. 68, pp. 197- 203 ,(1995) , 10.1080/09553009514551101
Stefan Winter, DNA-binding and fluorescence properties of the DNA bis-intercalating purple oxazole dimer POPO-1 Journal of Biomedical Optics. ,vol. 2, pp. 125- 130 ,(1997) , 10.1117/12.260019
G. T. Hirons, J. J. Fawcett, H. A. Crissman, TOTO and YOYO: new very bright fluorochromes for DNA content analyses by flow cytometry. Cytometry. ,vol. 15, pp. 129- 140 ,(1994) , 10.1002/CYTO.990150206