An enzyme immunoanalytical system based on sequential cross-flow chromatography.

作者: Joung-Hwan Cho , Eui-Hwan Paek , Il-Hoon Cho , Se-Hwan Paek

DOI: 10.1021/AC048270D

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摘要: A new enzyme immunoanalytical concept that can be used for point-of-care testing has been investigated. Enzyme as a tracer requires separate reaction step signal generation, which follows the completion of immune complex formation with analyte (e.g., Hepatitis B surface antigen) in sample. This major factor limiting its utilization within laboratory. We carried out such sequential processes employing chromatographic analysis, using two crosswise-arranged membrane pads vertical and horizontal directions. The vertically arranged were same those usual format pregnancy testing, instance, exception use horseradish peroxidase (HRP) tracer. By placing horizontally on each lateral side generation pad arrangement, they employed to supply substrate present complexes. flow was initiated after antigen−antibody bindings produce signal, ...

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