The barley powdery mildew protein kinase C gene, pkc1 and pkc-like gene, are differentially expressed during morphogenesis.
作者： Zhang Ziguo Zhang Ziguo , G Priddey , SJ Gurr
DOI: 10.1046/J.1464-6722.2001.00081.X
关键词:
摘要: summary Protein kinase C agonist assays revealed the phorbol ester, 12-myristate 13-acetate, invoked germling morphogenesis and enhanced PKC activity in Blumeria graminis. No antagonist of mildew was found but data fuelled a hunt for powdery pkc genes. Oligonucleotides, designed on basis conserved ATP-binding domains within catalytic core eukaryotic protein proteins, were used as primers to amplify chromosomal cDNA fragments from barley fungus Three gene isolated (pkc1, pkc-like cpka) full length genomic sequences genes determined by 'step down' PCR. RT-PCR transcript profiles showed three be differentially regulated during morphogenesis.
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