PKCu is a novel, atypical member of the protein kinase C family.
作者： F.J. Johannes , J. Prestle , S. Eis , P. Oberhagemann , K. Pfizenmaier
DOI: 10.1016/S0021-9258(17)37580-4
关键词:
摘要: We have isolated the full-length cDNA of a novel human serine/threonine protein kinase gene. The deduced sequence shows strong homology to conserved domains members C (PKC) subfamily. Homologies reside in duplex zinc-finger-like cysteine-rich motif and domain. lack C2 domain Ca(2+)-dependent PKCs presence unique NH2-terminal with potential signal peptide transmembrane suggest that PKC mu is member subgroup atypical PKCs. An open reading frame coding for 912 amino acids directs an vitro translation product apparent M(r) 115,000. In phorbol ester binding studies assays lysates cells overexpressing showed ester-independent activity, autophosphorylation, and, normal rat kidney (NRK) cells, predominant phosphorylation 30-kDa at serine residues. Southern analysis revealed single copy gene located on chromosome 21. There constitutive low level expression tissues transcript 3.8 kilobases elevated levels selected tumor cell lines. These data role transduction pathways related growth control.
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