Inhibition of advanced glycation end-products protects against retinal capillary basement membrane expansion during long-term diabetes

摘要: The purpose of this study was to investigate the advanced glycation end-product (AGE)-inhibitory properties aminoguanidine and determine whether treatment in long-term diabetic rats can prevent basement membrane lesions retinopathy. Four groups male Wistar were studied: untreated diabetics injected with 45 mg/kg streptozotocin, aminoguanidine-treated diabetics, controls, controls. After 12 months' diabetes, retinas from six animals processed for electron microscopy or retinal microvasculature isolated using trypsin digest technique. Stereological analysis used estimate quantitative ultrastructural changes capillary-associated membrane. Serum AGEs quantified by competitive AGE-ELISA, while microvascular-associated, immunoreactive analysed on digests. Aminoguanidine significantly reduced serum group (p < 0.001). In capillaries, there a marked reduction AGE immunoreactivity when compared diabetics. surface area absolute volume capillary increased non-diabetic controls 0.001 p 0.001, respectively). protected against expansion counterparts. prevents development diabetes-induced capillaries. inhibition suggest that play an important role complex pathogenesis thickening during