Altered Proliferation of Retinal Microvascular Cells on Glycated Matrix

摘要: Purpose. To investigate the effect of nonenzymatic glycosylation (glycation) basement membranes (BM) and isolated BM proteins on growth retinal pericytes endothelial cells. Methods. Type IV collagen, laminin, Engelbreth-Holm-Swarm tumor membrane (EHS-BM) bovine (RBM), after incubation in presence reducing sugars to induce glucose-mediated modifications, or absence any sugar (control), were used as a substrate culture microvascular Cell nonenzymatically glycosylated corresponding control substrates was measured daily, using an automated cell counter. Results. Retinal seeded glycated type collagen proliferated consistently more slowly than (P = 0.02), showing 20% 33% decrease throughout most curve, whereas laminin difference from not significant. In contrast, proliferation increased by 16% 25% for cells compared with 0.025), curve significantly different control. When whole EHS-BM RBM, decreased 30% 0.04); showed no EHS-BM, however, rate RBM it did 0.01). Conclusions. Nonenzymatic intact individual macromolecules resulted reduced These vitro observations resemble some pathologic changes observed situ, when diabetic retinopathy develops. Invest Ophthalmol Vis Sci. 1995;36:2358-2367. JLJiabetic is among common complications one leading causes vision loss industrialized countries. The prevalence strongly associated duration diabetes. Epidemiologic studies have concluded that ophthalmologically apparent develops 50% 98% persons diabetes 15 years diagnosis 1 ' 2