Changes in protein abundance and activity induced by drought during generative development of winter barley ( Hordeum vulgare L.)

摘要: Abstract The present study investigated drought-induced changes in proteome profiles of ten DH lines winter barley, relatively varied water deficit tolerance level. Additionally, the parameters describing functioning photosynthetic apparatus and activity antioxidative system were analysed. Water (3-week growth soil with content reduced to ca. 35%) induced significant leaf relations activity, probably due decreased stomatal conductance. It was associated protein abundance altered enzymes. From 47 MS-identified proteins discriminating more tolerant from drought-sensitive genotypes, only two revealed distinctly higher while seven lower drought-treated plants comparison sensitive ones. majority involved dark phase photosynthesis. Another factor great importance seems be ability sustain, during drought stress, high enzymes (SOD CAT) decomposing reactive oxygen species protecting plant cell oxidative damages. Low molecular weight antioxidants seem play less important roles. Our findings also suggest that stress barley is a constitutively controlled trait regulated by rate synthesis their Biological significance According our knowledge, this first comparative proteomic analysis performed for model set several doubled haploid (DH) lines. We analysed both impact on pattern individual as well comparisons between them according level tolerance. have identified drought-tolerant genotypes. opinion constitutively-controlled