Analysis of oligomannuronic acids and oligoguluronic acids by high-performance anion-exchange chromatography and electrospray ionization mass spectrometry.
作者： Yan Liu , Xiao-Lu Jiang , He Cui , Hua-Shi Guan
DOI: 10.1016/S0021-9673(00)00324-1
关键词:
摘要: Oligomannuronic acids and oligoguluronic were prepared by enzymatic hydrolysis of alginate with lyases. The oligosaccharides generated up to degree polymerization 16 characterized high-performance anion-exchange chromatography (HPAEC) pulsed amperometric detection (PAD) electrospray ionization mass spectrometry (ESI-MS). Acetate buffer linear gradients used as mobile phases for separations oligosaccharides. ESI-MS HPAEC-PAD are very effective the analysis characterization anionic
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