In vitro gastrointestinal digestion study of two wheat cultivars and evaluation of xylanase supplementation

摘要: The filamentous fungus Talaromyces versatilis is known to improve the metabolizable energy of wheat-based poultry diets thanks its ability produce a pool CAZymes and particularly endo-β(1,4)-xylanases. In order appreciate their in vivo mode action, supplementation effect two xylanases, XynD XynB from families GH10 GH11 respectively, have been evaluated on different wheat cultivars Caphorn Isengrain, which were chosen amongst 6 varieties for difference non starch polysaccharides content arabinoxylan composition. Polysaccharides digestion was followed during 6 h along digestive tract using TNO gastrointestinal model-1, mimic monogastric metabolism. Polysaccharide degradation appeared occur mainly at jejunal level higher with Isengrain than Caphorn. For both cultivars, increased notably amount reducing end sugars into jejuno-ileal dialysates, has confirmed by valuable increase soluble glucose dialysates. amounts arabinose xylose dialysates ileal deliveries consequently Caphorn, suggesting that diet could alleviate anti-nutritional effects arabinoxylans limiting physical entrapment available energy.